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We also examined the Firm of genes that specify adhesins in the chaperone/usher loved ones in other germs. We noticed that a gene cluster in Y. pestis
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Amongst the number of biofilm-defective mutants acquired, just one was influenced inside of a gene encoding a protein similar to periplasmic chaperones from the chaperone/usher pathway in a number of microorganisms (13). This gene belongs into a cluster that we identified as cupA
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To show that the whole chaperone/usher pathway is linked to biofilm development, and the nonadherent phenotype is not really exclusively connected with a defect inside the CupA2 chaperone, we released a mutation while in the gene encoding the usher component (